White clover (Trifolium repens L.) is a stoloniferous and perennial species that is the major forage legume oflivestock systems in temperate regions of the world. The objectives of the study were to determine nuclear DNA content and ploidy level of the native white clover populations growing in natural pastures of the Tekirdag region, Turkey. Nuclear DNA content analysis was carried out by flow cytometer using propidium iodide (PI) as a fluorochrome. The mean 2C nuclear DNA content of clover populations analysed in the study varied between 2.307 ± 0.063 and 2.345 ± 0.048 pg. Differences among the clover populations were not significant statistically. Based on the results of the study, all white clover genotypes investigated in the study were determined as tetraploid with 2n = 4x = 32 chromosomes.