Japanese flowering cherry (Prunus serrulata Lindl. cv. Kanzan) tree is one of the most popular ornamental plant species with light pink double flowers having unfolded petals from crimson buds. It is recommended for landscape architecture and gardening arrangements because of its mass floral display in spring. P. serrulata Kanzan is grown as shade trees in home gardens, parks, and on the roadsides in the Trakya region as well as in Tekirdağ province of Turkey.  In 2019, some systemic disease symptoms such as mild diffuse chlorotic mosaic and slightly chlorotic local spots on leaves of some P. serrulata trees in Tekirdağ have been observed. To identify PPV, 15 symptomatic and asymptomatic leaf samples were collected from different P. serrulata trees growing on the roadside and in-home gardens, and screened by RT-PCR test. Two out of the fifteen samples were found infected with Plum pox virus (PPV). The amplicon of one positive sample was sequenced, and a partial sequence was submitted in the GenBank. Analysis of the obtained sequence revealed that it had 100-44.30% nucleotide and 100-10.97% amino acid identities of PPV isolates deposited in the GenBank from diverse origins of Prunus species worldwide. PPV is a causal agent of Sharka disease on stone fruits and ornamental Prunus trees. Until now, no information has been available about the occurrence as a natural host of PPV on P. serrulata Kanzan in Turkey. To our best knowledge, this is the first report of PPV on P. serrulata Kanzan in Turkey.